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Nicotinamide-N-methyltransferase controls behavior, neurodegeneration and lifespan by regulating neuronal autophagy

2011 Mar;110(3):774-780

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At predetermined time intervals, aliquots were withdrawn and replaced with fresh buffer

This concentration allows precise dosing in 100mcg increments using standard insulin syringe markings (each 0.02mL or 2-unit increment equals 100mcg), which is critical for research protocols involving dose titration in the 250-1000mcg range

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