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Collagen & Structural Support: GHK-Cu supports collagen production and skin health, while BPC-157 and TB-500 contribute to connective tissue strength and repair

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University of Illinois Urbana-Champaign

In-vitro studies show both GHK and GHK-CU increase fibroblast activity that enhances the production of collagen, elastin, fibronectin, and other extracellular matrix components

Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

Effect of thiols on the reductive activation rate