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We initiated an evolution campaign with seven PANCE passages on GD_M, followed by 12 passages of high-stringency dual selection penalizing proteases that cleave VAMP1, and concluded with 16 passages on a circuit using a weaker promoter in front of the GD_M substrate to increase positive selection stringency (Extended Data Fig
10.1093/ndt/gfh932 154 LeyJ
However, its synthetic derivative has been shown in clinical studies to be of benefit to other organ systems in small doses
Researchers observed that intra-articular administration was associated with changes in cartilage matrix composition and chondrocyte activity markers in the treated joints compared to controls [3]
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