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SARS-CoV-2 virulence factor ORF3a blocks lysosome function by modulating TBC1D5-dependent Rab7 GTPase cycle

( mRNA quantification by quantitative real-time PCR (qRT-PCR) Total RNA was isolated using QIAzol-Reagent (Qiagen, Germany), based on modified Chomczyski & Sacchis method (Chomczynski and Sacchi, 2006), following the manufacturers protocol

Consistency: Look for regular, ongoing testing rather than one-off results

Review of the metabolism of 2-hydroxy-4-(methylthio) butanoic acid