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Approximately, half of the pelleted cells were lysed by incubation with 120 l CHAPS (Merck Millipore, including 0.15 units/l RiboLock [LifeTechnologies, Thermo Fisher Scientific]) on wet ice for 30 min and three short vortexes, thereafter the lysate was stored at 80 C

In vivo studies on urolithin A and spermidine Even though both UroA and spermidine can affect autophagy and mitophagy, in vivo studies with UroA predominantly focus on mitophagy, while those with spermidine focus more on autophagy

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