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Resulting concentration: 100 mg/mL
Equal amounts of protein lysates were loaded on Novex WedgeWell Tris-glycine or Bis-Tris gels (ThermoFisher) and blotted onto nitrocellulose or PVDF Trans-Blot Turbo membrane (0.2 m, Bio-Rad) according to standard protocols
Fifty clones were pooled, expanded and used for experiments
Nature Reviews Clinical Oncology
Findings from further research showed that the TME of the high-risk group had reduced CD8 + T cells and increased expression of immune checkpoint genes (such as PD-1 ), increasing immune escape (97)
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