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Images were acquired using the Leica DM6000B fluorescence microscope

Most protocols target the middle of this range

GSTZ1-1 is a bifunctional enzyme that, acting as maleylacetoacetate isomerase, is also involved in the metabolic degradation of phenylalanine and tyrosine 83

The reaction mixtures were manually mixed using a 100 to 300 l multichannel pipette (set to 250 l) and the microplate was inserted into a BioTek H1 Synergy microplate reader equilibrated to 37 C and vigorously shaken for 1 min followed by a 3.5-min delay

Bruckbauer 2013