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Cells (3 10 5 /well) were seeded in 6-well plates, then place the plates into the IncuCyte Live-Cell Analysis System (ESSEN BIOSCIENCE)
756 Overexpression of ACSL4 exacerbates cerebral I/R injury in rodents, while genetic or pharmacological reduction of ACSL4 effectively prevents cerebral I/R injury
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Membranes were washed with PBST, blocked with 5% non-fat milk, and incubated with the antibodies indicated (Supplementary Table 2)
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The first enzyme is glutamate cysteine ligase
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