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Lysis buffer: Tris-HCl, pH 7.4, 125 mM, NaCl 150 mM, DTT 1 mM, EDTA 1 mM, Lysozyme 1 mg/mL, Triton X-100 1 % (v/v), Protease inhibitor 1x Wash buffer: Tris-HCl, pH 7.4, 125 mM, NaCl 150 mM, DTT 1 mM, EDTA 1 mM

The relationship of permeability to octanol-water partition coefficient and molecular weight was found to be predictable for drugs with molecular weights less than 400 daltons

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