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These results indicate that many mutations acquired during early rounds of PANCE and dual selection were not essential for gasdermin D cleavage and, in some cases, were detrimental to protease efficiency in the context of GD_L variants
10.1021/acs.jafc.1c03361 26 DannenmannB.LehleS.HildebrandD
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& Jorns, M
H., Jung, Y
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