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This strict requirement exists because food, beverages, and other medications significantly reduce semaglutide absorption from the gastrointestinal tract

All participants underwent a 5-day modified fasting therapy introduced with 2-day moderate calorie restriction

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K48R and D77 mutations were introduced to prevent undesirable Ub chain elongation, and the K29R mutation was introduced to restrict the K29 linkage position in the second reaction step (Supplementary Fig