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The resulting decrease in antioxidant capacity further exacerbates oxidative stress and neuronal vulnerability in HD [166]
KT QU CHO THY : Da sng ln ng k sau 12 tun b sung L-Cystine kt hp vi L-Glutathione bng ng ung
The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)
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( e ) Cells expressing empty vector (EV) or CCNE1 were treated with the IDH1 inhibitor or the PARP inhibitors veliparib (left) or niraparib (right) alone or in combination (n = 4 technical replicates representative from 2 independent experiments in each cell line)
PMID 21593213
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