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Based on the amount of GSH in the lysate, we calculated the GSH concentration assuming an averaged HeLa cell volume of 4,000 m 3 (refs 15, 51, 52)
Another peptide, GHK-Cu, is being studied for potential cosmetic benefits such as for photoaging and hair loss and was added to the Category 1 list (but not for injectable routes of administration)
Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) For in vitro experiments, cell lysis and cDNA synthesis were performed using the SuperPrepII cell lysis & RT kit (Toyobo, Osaka, Japan) according to the manufacturer's instructions
Takahashi N, Harada M, Hirota Y, et al
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