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After baseline subtraction (() control), the initial ATPase activity rate was determined via simple linear regression (linear range, GraphPad Prism 10) of time-dependent absorbance measurements and normalized to unmodified HpGroEL activity (100%)

doi: 10.1210/en.2017-00461 97 LigumskyHWolfIIsraeliSHaimsohnMFerberSKarasikAet al

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Population-level anthropometric and metabolic characteristics are relatively stable over short time intervals, supporting the application of contemporary population data to evaluate trial eligibility criteria from this period