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RNA isolation and library preparation for RNA-seq RNA isolation was performed as previously described with some modifications 74

These findings do not constitute proof of efficacy in humans

6 Perspectives Though the knowledge regarding gut microbiota, metabolism and immunity has increased in fish and shrimp in recent years, several issues still merit further investigations

Conversion of cholecalciferols to calcidiol is dependent solely upon substrate availability as there is no regulatory mechanism to control the formation of calcidiol