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Quantitative real-time PCR (RT-qPCR) For the RT-qPCR analysis, total RNA was isolated from cells, and cDNA was reverse-transcribed by using M-MLV reverse transcriptase (Promega, Madison, WI, USA)
Retention time and spectral identity confirmed through chromatographic analysis
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First, by stimulating lipolysis a.k.a
Researchers interested in peptide handling, storage recommendations, HPLC analysis, LC-MS verification, and laboratory best practices are encouraged to visit our Research Peptide Guides
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