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doi: 10.1016/j.jtcme.2020.03.005
264 SIRT5 and SIRT6 are master regulators involved in metabolic reprogramming during tumorigenesis
Repeated freeze-thaw cycles should be avoided to preserve the integrity of the copper-peptide complex and ensure consistent experimental results
75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)
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