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The supernatant was discarded and samples were resuspended in 5 Laemmli buffer
Its use is strictly limited to in vitro laboratory work (like cell cultures) or preclinical in vivo studies in animal models
Nampoothiri S, Nogueiras R, Schwaninger M, Prevot V
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However, futile quinone redox cycling can lead to a cascade of ROS formation and as such, the link between toxicity and quinone redox cycling is widely acknowledged 5
In cases with borderline low serum cobalamin levels, measuring serum methylmalonic acid (MMA) and fasting homocysteine levels may clarify whether these are truly low-normal values or whether they instead indicate a physiologically significant functional vitamin B12 deficiency
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