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Estimated response and change in response analyses used the baseline measure in question (HbA 1c , body weight, DTSQs), age and baseline BMI as covariates, and sex, oral antidiabetics at baseline, diabetes duration and site as fixed factors

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In total, 50 L Binding buffer (30% ACN in 200 mM Ammonium acetate, pH 4.5) was added to 50 L of protein solution for a final volume of 100 L

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The C1 protocol consisted of the thermal treatment of the fermented product and the biomass at 80 C for 30 with the aim of heat-inactivating the bacterial cells