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Changes to the microbial phyla and genera observed in the 5A-1MQ-treated group switched from WD to LD could have been driven by additive, synergistic, or independent mechanisms relative to those induced by diet switch alone, which could not be determined with the current study design that lacked a group treated with 5A-1MQ and maintained on WD

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5A-1MQ-treated mice switched from WD to LD (WD/LD-T) showed a rather reduced relative abundance of Akkermansia compared to vehicle-treated DIO animals switched from WD to LD (WD/LD-V), suggesting a unique microbiome mechanism associated with weight loss in the vehicle-treated group switched from WD to LD compared to the 5A-1MQ-treated group that underwent the same diet switch

References Kommu, S., & Whitfield, P