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This method is based on the color reaction between allyl thiourea and potassium ferricyanide solution in dilute acetic acid which measures the optical density of the color complex at 600 nm and it works in the range of 2.5 to 7.5 picogram of AITC per milliliter of the color solution

Its hard to use a different method once you try the different method

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Primacy of the 3B approach to control risk factors for cardiovascular disease in type 2 diabetes patients

Dreaden EC, et al

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