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In contrast to class I GRXs, which are glutathionylated at Cys A over a wide range of GSH/GSSG redox potentials, only the internal disulfide-containing oxidized form is detected for ROXY9 at a midpoint redox potential between 220 and 230 mV at p H 7.0
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Cells were washed with PBS and centrifuged simultaneously at 1000 rpm
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