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Medium was exchanged to vascular perfusion medium (VPM) comprising Medium 199 (Biozym, Hessisch Oldendorf, Germany), 5% (v/v) human autologous serum, 680 M L-glutamine, 25 g/mL heparin (Sigma-Aldrich), 5 g/mL ascorbic acid (Thermo Fisher Scientific), 10 ng/mL M-CSF, 10 ng/mL GM-CSF and AAS prior to the seeding of MDMs

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