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Cells were digested and collected in a new EP tube and fixed them with cold ethanol at 4C overnight

Persistent factors, including high-temperature exposure, prolonged sedentary behavior, varicocele, and cryptorchidism, can elevate local testicular temperature, disrupt the spermatogenic microenvironment, and thus impair male fertility

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Ipamorelin mimics ghrelin and triggers the immediate release of growth hormone

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