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Further mechanism studies showed that MDG-1 promoted the expression of sphingosine kinase 1 and sphingosine-1-phosphate (S1P) receptor 1, induced the expression of basic fibroblast growth factor (bFGF) in HMEC-1 cells, and induced Akt and extracellular signal-regulated kinase (ERK) phosphorylation in a dose-and time-dependent manner, thus protecting cardiomyocytes from ischemia-induced damage (58)

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Panel A shows the 95th percentile (blue), the mean (green), and the 5th percentile (red) of estradiol during the menstrual cycle