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Digested samples were desalted using a C18 spin column (Pierce C18 Spin, #89873) according to manufacturer instructions prior to resuspension in EPPS buffer and CH 3 CN for TMT labeling as described above

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The inactive thyroid hormone T4 is ultimately transformed into its active form T3, through the activation of the BA-TGR5-cAMP-DIO2 signaling pathway