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The resulting linear fragments were assembled using Gibson assembly (16 h, 50 C), purified using a DNA Clean and Concentrator kit (Zymo, D4004), and transformed into cells bearing pScreen-S1.3 (for IR03 and IR16) or pScreen-R2.1 (for IR02 and IR35) via electroporation

This conformational shift impairs the conversion of phosphoenolpyruvate (PEP), leading to the accumulation of glycolytic intermediates that fuel anabolic biosynthesis [96]

In addition, centrilobular necrosis, cellular hypertrophy and vacuolization were observed (Figures 3C, D)

and evaluated Cymbiotika within the broader wellness landscape.

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