The following primers were used, with GAPDH used as a control: SOD2 (Forward (F) - 5 ACGTCACCGAGGAGAGTACC 3, Reverse (R) - 5 TAGGGCTGAGGTTTGTCCAG 3), XIAP (F - 5 ATTCACTTGAGGAGTGTCTGGT 3, R - 5 ATGTCCTTGAAACTGAACCCCA 3), TNFRSF10A (F - 5 AGCCCTTTGGGAGTTGTG 3, R - 5 TGGTGCAGGGACTTCTCTCT 3), TNFRSF10B F - 5 GATTCAGAGCTCACAACGACC 3, R - 5 GGTGGGCTGCAAGATACTCA 3) and GAPDH (F - 5 GACAGTCAGCCGCATCTTCT 3, R - 5 GCGCCCAATACGACCAAATC 3)
Understanding these energy metabolism effects is relevant to research on obesity and metabolic health
Each production lot is independently characterized using high-performance liquid chromatography (HPLC) and liquid chromatographymass spectrometry (LCMS) protocols
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This is because of its FDA approval and 10+ year safety profile as well as multiple proven benefits for metabolism and cardiovascular risk with speculative/accumulating evidence for dementia prevention