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While controlled ROS levels are essential for normal sperm processessuch as capacitation, motility, and the fertilization mechanismexcessive ROS accumulation leads to oxidative damage, impairing sperm function and ultimately contributing to male infertility

Researchers commonly confirm that the reconstituted solution retains its characteristic blue tint, since a loss of color can indicate that an incompatible solvent or extreme pH has disrupted the copper coordination

Their nonspecific distribution also causes toxicity in organs such as liver, kidneys, lungs, and heart (van Erven and Schalij 2010)