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Third, applying the method in the microplate reader is challenging due to the requirement for protein precipitation
Shaking generates shear stress and an air-liquid interface that promote peptide aggregation, and the resulting foam traps material out of solution
Wayner DD, Burton GW, Ingold KU, Locke S
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Biomedicines 10(6):1254 Montero D, Walther G, Perez-Martin A, Roche E, Vinet A (2012) Endothelial dysfunction, inflammation, and oxidative stress in obese children and adolescents: markers and effect of lifestyle intervention
No long-term data specifically addresses this question for peptide nasal sprays
