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Technical factors also influence interpretation: NF-B activity is best judged by its nuclear translocation or phosphorylation, and IHC for total NF-B may overrepresent localized inflammatory foci 40 , and platelets can promote thrombo-inflammatory interactions with neutrophils, monocytes, and T cells during ischemiareperfusion injury

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Primers used for quantitative RT PCR analysis are included in Supplementary Data S6

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