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The enzyme phospholipase D hydrolyzes the phosphatidylcholine directly from the neuronal cell membrane, liberating free choline into the cytoplasm so it can be immediately converted into acetylcholine by the enzyme choline acetyltransferase
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Current gene therapy research aims to target, for example, upstream targets of the GSH system or molecules associated with it (such as Nrf2, GPX, SLC7A11), genes aberrantly expressed in myocardial injury, and noncoding RNAs regulating mRNA transcription [76, 202, 250,251,252,253]
It is also noteworthy that the combination of MCT4 inhibitors with anti-PD-1 immunotherapy significantly improved the outcome of HCC patients (137)
