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Activated sirtuins can improve sperm longevity and viability by promoting DNA repair mechanisms and regulating inflammatory responses within the reproductive tract

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Both lines, compatible with C57BL/6 mice, were maintained at 37 C in 5 % CO 2 in RPMI-1640 supplemented with 10 % fetal calf serum, 2 mM L-glutamine, 100 IU ml 1 penicillinstreptomycin, 1 mM sodium pyruvate and MEM non-essential amino acids