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After thorough mixing, 150 L of Milli-Q water was added, and 300 L of the mixture was filtered by centrifugation through a 5-kDa cutoff filter (ULTRAFREE MC PLHCC

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Then, in 2015, Lee and colleagues identified a previously overlooked open reading frame within the 12S ribosomal RNA gene of mtDNA and found that it encodes a functional peptide, MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA-c) [1]

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