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(49) and Jamil et al

The media collected from the spheroids were transferred to a clean microplate and incubated with equal volume of Griess Reagent on shaker at room temperature for 10 min to allow the Griess reaction to occur

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dorsalis , the total proteins from 100 nonviruliferous, viruliferous, or dsRNAs treated intact insects were extracted and detected by western blot assay with Pns11-, P8-, Pelo-, Hbs1-, E3-, Hsp70-, or H3 IgG as the primary antibody and goat anti-rabbit IgG-peroxidase (Invitrogen, 32460) or goat anti-mouse IgG-peroxidase (Invitrogen, 32430) as the secondary antibody