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Nonspecific reactivity was blocked and sequentially incubated with primary rabbit anti-TBR1 (1:500, Abcam), rat anti-Ctip2 (1:500, Abcam), mouse anti-SATB2 (1:400, Abcam) and mouse anti-BrdU (1:500) (Sigma-Aldrich).The slides were transferred to secondary antibodies labeled with Alexa Fluor 488 and 594 (Jackson ImmunoResearch Inc.) and covered with an anti-fade mounting media with DAPI
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