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Cecal samples Cecal weight was measured since the weight of this portion of the intestine appears to be highly influenced by the microbiome (e.g., certain antibiotic treatments dramatically increase cecal weight 27,28,29,30,31 but not the colon 27 )

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Normalized RNA-sequencing data (FPKM) were log2-transformed, and a folate metabolism signature score was derived based on the expression levels of these genes