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In vitro studies further validated these findings, showing that plasma from inflamed patients (e.g., PD patients with peritonitis) induces eryptosis in healthy donor RBCs
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The transfection protocol was consistent with previous reports ( 2.13 Western blot Proteins were extracted from colon tissue and cell samples and stored at 20C
Women who had preexisting diabetes, high fasting glucose level, a high two-hour glucose level, or contraindications to participation as determined by local investigators were excluded
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The diagnostic criteria for IC/BPS, based on the proposed guidelines of the European Society for the Study of Interstitial Cystitis, constituted chronic pelvic pain, pressure, or discomfort perceived to be related to the urinary bladder accompanied by at least one other urinary symptom, such as persistent urge to void or urinary frequency, for more than 6 months, and the exclusion of potentially similar diseases 11,25
