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Isolation and Storage of Peripheral Blood Mononuclear Cells, Plasma, and Red Blood Cells Peripheral Blood Mononuclear Cells (PBMCs), plasma, and red blood cells (RBCs) were isolated from whole blood by density gradient centrifugation using ficoll histopaque (Sigma, St

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POMC-derived peptides including adrenocorticotropin (ACTH), alpha melanocyte stimulating hormone (-MSH), -MSH and -endorphins, MC1R, adrenergic and cholinergic agents, and growth factors, cytokines, or nitric oxide (NO) secreted in the local microenvironment are all involved in the regulation of melanogenesis [6]

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We starved cells of cystine and supplemented the media without or with cystine for 3 h