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The media was then removed and the formazan crystals that formed during incubation were dissolved in 1 mL DMSO and processed for OD reading

In MA-HTC, biomass is heated to 180250 C under moderate pressure (210 MPa) for a short period (a few minutes to a few hours), leading to multiple chemical reactions, including dehydration, decarboxylation, hydrolysis, polymerization, polycondensation, and aromatization (Asomaning et al

Importantly, differences in findings between ME/CFS and long COVID suggest that the underlying neurobiological mechanisms, while leading to similar clinical presentations, may differ