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Interestingly, we transfected 293T cells with Myc-labeled ubiquitin, HA-labeled KEAP1, and Flag-labeled NRF2 plasmids and observed that the upregulation of KEAP1 promoted the poly-ubiquitination of NRF2, and SP could down-regulate the KEAP1 expression and block the NRF2 ubiquitination dose-dependently (Supplementary Fig

H2DCFDA fluorescence (FL-1) in propidium iodide-negative cells (FL-3) was tested by a FACSCalibur Flow Cytometer

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During this time, pilots must monitor for adverse effects and demonstrate that the medication is well tolerated