To explore upstream regulatory mechanisms, we identified all significantly expressed transcription factors (TFs) across exposures and brain regions in a sex-wise manner using GeneCard annotations (Figs
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LCMS-Based Metabolomics Cells were processed using the LIMeX workflow, and polar metabolites were extracted with a biphasic solvent system composed of cold methanol, methyl tert-butyl ether, and 10% methanol, followed by liquid chromatography-mass spectrometry (LCMS) analysis [45]