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Supplies: 3 mL syringe to mix and inject HCG Trigger, 22 gauge 1 inch needle to mix HCG Trigger, 27 gauge inch needle to inject HCG Trigger, alcohol swabs

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Instead, insert the needle at a 45-degree angle into the peptide vial's rubber stopper, then tilt the vial so the needle tip touches the glass wall

After sugar depletion in the initial medium containing 4% (v/v) molasses and 5% (v/v) CSL, a feeding solution of 50% (v/v) molasses and 25% (v/v) CSL was continuously added to the bioreactor

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