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Statistics and reproducibility Statistical significance was determined using independent t -test for immunohistochemical and western blot analyses, and one-way analysis of variance for cell cycle and apoptosis detection by GraphPad Prism 7.0 software (GraphPad, Inc., La Jolla, CA, USA)

J Genet Eng Biotechnol 2021

We identified 48 protein spots in 2-D gel analysis and selected proteins were divided into 64% collagen components and 30% non-collagen components as shown by the MALDI-TOF analysis

Some Risk: Confirmed use of alcohol during pregnancy with use less than High Risk or unknown usage patterns

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Apart from regular check-ins with your doctor or a weight loss clinic, you can also manage your lifestyle to achieve better results