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However, the role of neutrophil ferroptosis in immunodeficient diseases is worth considering, particularly whether there are shared pathways that mediate the process of ferroptosis

Acknowledgements This work was financially supported by the National Natural Science Foundation of China (Nos

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The following FBXO3 crRNA guides were ordered from IDT and used: sgFBXO3-3: GGAGGATTCCAGCAGAGACA sgFBXO3-4: TATCAAGTCATGATCCGCTG sgFBXO3-5: TGTTCATACCGAATTCACAA sgFBXO3-6: GACGTCGATACAGCTGCCGG Endpoint HiBiT protein degradation HiBiT reporter cells, generated as described above, were seeded into 384-well plates at 20,000 cells per well and treated with the indicated compounds and equivalent concentrations of DMSO for the time indicated in the experiment, usually 3, 24 or 72 h