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Lipid peroxidation assay Lipid peroxidation staining assay was performed using BODIPY 581/591 C11 dye (Thermofisher, MA) as previously described 19

5 l of 10% trifluoroacetic acid (TFA, cas 76-05-1, Sigma Aldrich, #302031) was added to each sample to quench the reaction

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Studies observed improvements in coat appearance including reduced graying and improved fur density, suggesting effects on stem cell function and tissue regeneration capacity

Subgroup analyses suggest enhanced benefits in older adults and those with treatment-resistant depression

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