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Around 5 10 6 cells per 100-mm-diameter dish were transfected with the ecotropic packaging plasmid pCL-Eco (4 g) together with one of the following retroviral constructs (4 g): pMXs-Klf4, pMXs-Sox2, pMXs-Oct4 or pMXs-cMyc (obtained from Addgene) using Fugene-6 transfection reagent (Roche) according to the manufacturers protocol

It was only recognized in the late 1980s that desensitization of insulin secretion, which describes a state of decreased secretory responsiveness of -cell to the glucose stimuli, is a pathophysiological phenomenon of -cell function and not just an in vitro artifact 30

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doi:10.4103/0253-7613.91876

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