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Shot day was the part of the week I always struggled to plan for, so when I built my full 7-day plan I made sure the dose day and the day after had the lightest, gentlest meals on purpose
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After reverse transcription using the GEM-RT incubation protocol, cDNA was purified with DynaBeads MyOne Silane beads (Invitrogen, Carlsbad, CA, #37002D), amplified with PCR, and further purified with SPRIselect reagent (Beckman, Indianapolis, Indiana, #B23318)
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