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Keep your doctor informed about your health condition and medications to rule out any interactions/side effects

Cycle threshold (Ct) values of target genes were calibrated to the Ct for the corresponding genes obtained for samples from the control pCYC1-GAP1 strain and normalised to the geometric mean of the reference genes for relative quantification using the comparative Ct method [36]

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10.1016/S0002-9394(01)01365-4

The body makes its own choline, but levels can drop with age, toxic exposures, or chronic illness