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The resolved gels were transferred from the pre-cast SDS-PAGE gels to polyvinylidenefluoride (PVDF) membranes, and blocking was performed with 5% bovine serum albumin (BSA) in Tris-buffered saline (TBS-T) containing 0.05% Tween-20 and incubated with the primary antibodies: MOTS-c antibody (MOTSC-101AP, FabGennix)

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Microneedling the scalp before AHK-Cu application can substantially improve penetration

Other symptoms of vitamin B12 deficiency may include problems with balance, depression, confusion, dementia, poor memory and soreness of the mouth or tongue

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